Difference between revisions of "Part:BBa K1830002:Experience"

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<I>Group: TMMU-China-2016, Author: Yifei Lu, Summary: We characterized that the 27 amino acids long SPusp45 signal peptide part BBa K1654007, but not the BBa_K1830002 part is the right choice that can be used to secret proteins out of ''L. lactis''.</I>
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<I>Aberdeen_Scotland 2009 </I>
 
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The initial gel worked although the obvious confirmation was not possible due to the very small fragment size (49bp).But later it was confirmed following its usage by forming the part BBa_K182002 through the PCR gel analysis and the sequencing.The plasmid miniprep also worked.
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Revision as of 14:12, 15 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1830002

BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the L.lactis cell. It should be fused to the N-terminal or C-terminal of the protein of interest.

iGEMers who want to use the SPusp45, they should choose the BBa K1654007 part, we have verified that BBa K1654007 is functional in protein secretion.

Group

TMMU-China-2016

Author

Yifei Lu

Summary

We characterized that the 27 amino acids long SPusp45 signal peptide part BBa K1654007, but not the BBa_K1830002 part is the right choice that can be used to secret proteins out of L. lactis.

User Reviews

UNIQf45949aa0bc686f7-partinfo-00000000-QINU

••••

Aberdeen_Scotland 2009

The initial gel worked although the obvious confirmation was not possible due to the very small fragment size (49bp).But later it was confirmed following its usage by forming the part BBa_K182002 through the PCR gel analysis and the sequencing.The plasmid miniprep also worked.

UNIQf45949aa0bc686f7-partinfo-00000002-QINU