Difference between revisions of "Part:BBa K2060001"

Revision as of 14:10, 15 October 2016

Fluorescent protein mKeima expressed by Arabinose promotor

mKeima is a red-shifted fluorescent protein with Excitation of 440nM and Emission of 616nM. In this part mKeima expression is induced by Arabinose driven by the wildtype pBAD promotor. The red shifted emission spectrum gives it good potential for mammalian cell or tissue bioimaging.

Usage and Biology

We attempted to use arabinose to induce expression of this biobrick variety of concentrations and time periods. Our success with this expression are documented in the Experience section.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2060001 short</partinfo>
-mKeima is a red-shifted fluorescent protein with Excitation of 440nM and Emission of 616nM. In this part mKeima expression is induced by Arabinose driven by the wildtype [https://parts.igem.org/Part:BBa_I13453 pBAD promotor]. The red shifted emission spectrum gives it good potential for mammalian cell, tissue or animal research. https://www.ncbi.nlm.nih.gov/protein/AMO27260.1
+mKeima is a red-shifted fluorescent protein with Excitation of 440nM and Emission of 616nM. In this part mKeima expression is induced by Arabinose driven by the wildtype [https://parts.igem.org/Part:BBa_I13453 pBAD promotor]. The [https://www.ncbi.nlm.nih.gov/protein/AMO27260 red shifted emission spectrum] gives it good potential for mammalian cell or tissue bioimaging.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+We attempted to use arabinose to induce expression of this biobrick variety of concentrations and time periods. Our success with this expression are documented in the Experience section.
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