Difference between revisions of "Part:BBa K2012015"
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PcpcG2 promoter is a 238bp green-light activated promoter in Synechocystis PCC 6803(Part:BBa_K592003). The full length promoter is comprised of a G-box region, a CcaR-P activated promoter, and a constitutive promoter, which contributes to the leakiness under red light and low dynamic range. Therefore, we constructed PcpcG2-172, a 172bp truncated cpcG2 promoter deleted for the constitutive promoter.
 
PcpcG2 promoter is a 238bp green-light activated promoter in Synechocystis PCC 6803(Part:BBa_K592003). The full length promoter is comprised of a G-box region, a CcaR-P activated promoter, and a constitutive promoter, which contributes to the leakiness under red light and low dynamic range. Therefore, we constructed PcpcG2-172, a 172bp truncated cpcG2 promoter deleted for the constitutive promoter.
  
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<div style="margin-left:auto;margin-right:auto;position:relative"><p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri"><img height="288.0" src="https://static.igem.org/mediawiki/2016/a/ab/De.jpeg " width="480.0"></span><span style="font-family:Calibri"><span></span></span></p>
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<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">Fluorescence assay of CcaS-CcaR system with PcpcG2-238 and PcpcG2-172 in CL1 (△EnvZ), and PCB (△CcaS) as </span><span style="font-family:Calibri">chromophore</span><span style="font-family:Calibri">. <span></span></span></p>
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<p style="text-align:justify;text-justify:inter-ideograph"><a name="_GoBack"></a><span style="font-family:Calibri">&nbsp;</span></p>
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<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">Leaked expression in darkness significantly reduced after truncation of the constitutive promoter. By the way, the difference of fluorescence under green and red light is narrowed unexpectedly.</span><span style="font-family:Calibri;font-weight:bold"><span></span></span></p>
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<p><span>Fluorescence assay of CcaS-CcaR system withPcpcG2-238 and PcpcG2-172 in CL1 (</span><span style="font-family:宋体">△</span><span>EnvZ), and PCB (</span><span style="font-family:宋体">△</span><span>CcaS) as chromophore.</span></p>
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<p><span>Leaked expression in darkness significantlyreduced after truncation of the constitutive promoter. By the way, thedifference of fluorescence under green and red light is narrowed unexpectedly.<b></b></span></p>
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Revision as of 13:50, 15 October 2016


PcpcG2-172, a modified PcpcG2 promoter

PcpcG2 promoter is a 238bp green-light activated promoter in Synechocystis PCC 6803(Part:BBa_K592003). The full length promoter is comprised of a G-box region, a CcaR-P activated promoter, and a constitutive promoter, which contributes to the leakiness under red light and low dynamic range. Therefore, we constructed PcpcG2-172, a 172bp truncated cpcG2 promoter deleted for the constitutive promoter.


 

Fluorescence assay of CcaS-CcaR system with PcpcG2-238 and PcpcG2-172 in CL1 (△EnvZ), and PCB (△CcaS) as chromophore.

 

Leaked expression in darkness significantly reduced after truncation of the constitutive promoter. By the way, the difference of fluorescence under green and red light is narrowed unexpectedly.

 

Fluorescence assay of CcaS-CcaR system withPcpcG2-238 and PcpcG2-172 in CL1 (△EnvZ), and PCB (△CcaS) as chromophore.

 

Leaked expression in darkness significantlyreduced after truncation of the constitutive promoter. By the way, thedifference of fluorescence under green and red light is narrowed unexpectedly.

 



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]