Difference between revisions of "Part:BBa K1897012"

Revision as of 12:53, 15 October 2016

Listeriolysin O CDS

Listeriolysin O (LLO) is derived from Listeria monocytogenes and is a pore-forming toxin that allows L. monocytogenes to escape from the endocytic vesicle into the cytoplasm (Vadia et al., 2011).

Usage and Biology

LLO is essential for the pathogenesis of L. monocytogenes and is part of the family of the cholesterol-dependent cytolysis (CDCs). They have a size range of 50 to 70 kDa, and bind to a host receptor on the host membrane. Upon binding, they diffuse laterally to form a ring-shaped oligomeric prepre complex then then inserts a large β-barrel pore across the membrane in a cholesterol-dependent fashion (Vadia et al., 2011). Formation of the pore allows LLO to escape from the phagosomal compartment that it is in when it first enters a cell.

LLO also has a acidic pH optimum (around pH 5 to 5.5), which may thus explain why it is able to escape the phagosomal compartment that it is trapped in when it first enters the cell, but requires additional internalisation proteins for it to first enter a non-phagocytic cell (Kayal and Charbit, 2006; Vadia et al., 2011).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1380
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1897012 short</partinfo>
-Listeriolysin O (LLO) is derived from <i>Listeria monocytogenes</i> and is a pore-forming hemolysin ....... (add info)
+Listeriolysin O (LLO) is derived from <i>Listeria monocytogenes</i> and is a pore-forming toxin that allows <i> L. monocytogenes </i> to escape from the endocytic vesicle into the cytoplasm (Vadia <i>et al.</i>, 2011).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+LLO is essential for the pathogenesis of <i>L. monocytogenes</i> and is part of the family of the cholesterol-dependent cytolysis (CDCs). They have a size range of 50 to 70 kDa, and bind to a host receptor on the host membrane. Upon binding, they diffuse laterally to form a ring-shaped oligomeric prepre complex then then inserts a large β-barrel pore across the membrane in a cholesterol-dependent fashion (Vadia <i> et al.</i>, 2011).  Formation of the pore allows LLO to escape from the phagosomal compartment that it is in when it first enters a cell.
+LLO also has a acidic pH optimum (around pH 5 to 5.5), which may thus explain why it is able to escape the phagosomal compartment that it is trapped in when it first enters the cell, but requires additional internalisation proteins for it to first enter a non-phagocytic cell (Kayal and Charbit, 2006; Vadia <i>et al.</i>, 2011).
 <!-- -->