Difference between revisions of "Part:BBa K1951005"
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It has been demonstrated that Flagellin has the ability to adsorbe precious metal such as Platinum, gold.. | It has been demonstrated that Flagellin has the ability to adsorbe precious metal such as Platinum, gold.. | ||
| − | Codons have been optimized allowing a high transcription level. | + | === Design resum == |
| + | Codons have been optimized for <i> Escherichia coli </i> allowing a high transcription level. | ||
Every forbidden restriction sites have been removed and sequence is optimital. | Every forbidden restriction sites have been removed and sequence is optimital. | ||
| + | |||
| + | === Proof of fonctionnality === | ||
This sequence has been tested under the control of a strong promoter and <u>validated as fonctionnal</u>. [ | This sequence has been tested under the control of a strong promoter and <u>validated as fonctionnal</u>. [ | ||
Revision as of 12:35, 15 October 2016
FliC E.coli coding sequence
This coding sequence codes the Flagellin C (FliC) protein, filament of the flagellum from Escherichia coli strain.
FliC is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming. In the possession of flagellum, bacteria can move what confers them a selective advantage. It has been demonstrated that Flagellin has the ability to adsorbe precious metal such as Platinum, gold..
= Design resum
Codons have been optimized for Escherichia coli allowing a high transcription level.
Every forbidden restriction sites have been removed and sequence is optimital.
Proof of fonctionnality
This sequence has been tested under the control of a strong promoter and validated as fonctionnal. [
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1224
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
Illegal AgeI site found at 709 - 1000COMPATIBLE WITH RFC[1000]