Difference between revisions of "Part:BBa K2110005"

Latest revision as of 11:59, 15 October 2016

TPA inducible RFP expression system

PETase gene encodes the PETase enzyme which can degrade PET to terephthalic acid (TPA) and ethylene glycol (EG) with another enzyme MHETase. However, the product TPA can cause inhibition of the reaction and slow down it. We want to use TPA as inductor of the promoter and this part is used to test. This system consists of the TPA inducible Leader sequence(BBa_K2110001), the enhanced promoter PGK1(BBa_K2110012),the mRFP-1 gene(BBa_BBa_E1010) and Terminator CYC1(BBa_K2110000). It can be induced by product TPA. This system is used in Saccharomyces cerevisiae.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1446
Illegal AgeI site found at 1558
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2110005 short</partinfo>
-PETase gene encodes the PETase enzyme which can degrade PET to terephthalic acid (TPA) and ethylene glycol (EG) with another enzyme MHETase. However, the product TPA can cause inhibition of the reaction and slow down it. This expression system consists of the TPA inducible Leader sequence(BBa_K2110001), the enhanced promoter PGK1(BBa_K2110012), α-factor signal peptide(BBa_K2110017),the PETase gene(BBa_K2110800) and Terminator CYC1(BBa_K2110000). It can be induced by product TPA so that this is a positive feedback system. This system is used in Saccharomyces cerevisiae.
+PETase gene encodes the PETase enzyme which can degrade PET to terephthalic acid (TPA) and ethylene glycol (EG) with another enzyme MHETase. However, the product TPA can cause inhibition of the reaction and slow down it. We want to use TPA as inductor of the promoter and this part is used to test. This system consists of the TPA inducible Leader sequence(BBa_K2110001), the enhanced promoter PGK1(BBa_K2110012),the mRFP-1 gene(BBa_BBa_E1010) and Terminator CYC1(BBa_K2110000). It can be induced by product TPA. This system is used in Saccharomyces cerevisiae.
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