Difference between revisions of "Part:BBa K1897010"
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The sequence of invasin was obtained from Genebank (see source of invasin CDS under 'Design' page) and sent for synthesis after modifying the sequence to include a promoter, RBS, 2 HA tags and a double terminator (see <partinfo>BBa_K1897011</partinfo>). | The sequence of invasin was obtained from Genebank (see source of invasin CDS under 'Design' page) and sent for synthesis after modifying the sequence to include a promoter, RBS, 2 HA tags and a double terminator (see <partinfo>BBa_K1897011</partinfo>). | ||
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Latest revision as of 11:41, 15 October 2016
Invasin CDS
Invasin is derived from Yersinia pseudotuberculosis, and codes for a 103-kDa membrane surface protein that recognises and binds to β1-integrin on the surface of eukaryotic cells (Isberg and Leong, 1988; McCormick et al., 1997).
Usage and Biology
Invasin allows Yersinia to invade mammalian cells and hide from the immune system. Once invasin binds to β1-integrin on the surface of eukaryotic cells, it is internalised into the cell (Isberg and Leong, 1988). It has been shown by Isberg and Falkow (1985) that with just the invasin gene alone, Escherichia coli can invade mammalian cells expressing β1-integrin. This allows for the entry of E. coli into mammalian cells, and allows for potential delivery of molecules into targeted mammalian cells when used in conjunction with other systems (for example, with the use of quorum sensing, where only in an area of high bacterial cell density will invasin be produced).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2701
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 886
Illegal AgeI site found at 2395 - 1000COMPATIBLE WITH RFC[1000]
Uses of Invasin CDS
The sequence of invasin was obtained from Genebank (see source of invasin CDS under 'Design' page) and sent for synthesis after modifying the sequence to include a promoter, RBS, 2 HA tags and a double terminator (see BBa_K1897011).