Difference between revisions of "Part:BBa K2110101"

Revision as of 11:29, 15 October 2016

PETase site-directed mutant S207T

This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant. Sequence and Features

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 79
Illegal BglII site found at 289
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Results

We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.

We used the relative fluorescence units to represent the expression level of enzymes.

Finally we got the relative activities of enzymes.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2110101 short</partinfo>
-This is one of the site-directed mutant of the PETase gene, we change the 208th amino acid from I to V. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
+This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
 Sequence and Features