Difference between revisions of "Part:BBa K1897010"

Revision as of 11:09, 15 October 2016

Invasin CDS

Invasin is derived from Yersinia pseudotuberculosis, and codes for a 103-kDa membrane surface protein that recognises and binds to β1-integrin on the surface of eukaryotic cells (Isberg and Leong, 1988; McCormick et al., 1997).

Usage and Biology

Once invasin binds to β1-integrin on the surface of eukaryotic cells, it is internalised into the cell (Isberg and Leong, 1988). It has been shown by Isberg and Falkow (1985) that with just the invasin gene alone, Escherichia coli can invade mammalian cells expressing β1-integrin. This allows for the entry of E. coli into mammalian cells, and allows for potential delivery of molecules into targeted mammalian cells when used in conjunction with other systems (for example, with the use of quorum sensing, where only in an area of high bacterial cell density will invasin be produced).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 2701
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 886
Illegal AgeI site found at 2395
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-Once invasin binds to β1-integrin on the surface of eukaryotic cells, it is internalised into the cell (Isberg and Leong, 1988). It has been shown by Isberg and Falkow (1985) that with just the invasin gene alone, <i>Escherichia coli</i> can invade mammalian cells expressing β1-integrin. This allows for the entry of <i>E. coli</i> into mammalian cells, and allows for potential delivery of molecules into targeted mammalian cells when used in conjunction with other systems (for example, with the use of quorum sensing, where only in an area of high bacterial cell density will Invasin be produced.).
+Once invasin binds to β1-integrin on the surface of eukaryotic cells, it is internalised into the cell (Isberg and Leong, 1988). It has been shown by Isberg and Falkow (1985) that with just the invasin gene alone, <i>Escherichia coli</i> can invade mammalian cells expressing β1-integrin. This allows for the entry of <i>E. coli</i> into mammalian cells, and allows for potential delivery of molecules into targeted mammalian cells when used in conjunction with other systems (for example, with the use of quorum sensing, where only in an area of high bacterial cell density will invasin be produced).
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