Difference between revisions of "Part:BBa K1899004"

Revision as of 11:05, 15 October 2016

phlFp promoter

phlFp is a constitutive promoter. repressed by phlF repressor (BBa_K1725040) induced by 2,4-Diacetylphloroglucinol ( DAPG ) inducer.

Results

Experiments on comparing the strength of phlFp, tetp and lacp, were performed. Results indicated that phlFp is the strongest among the three, with 1.96 times and 6 times stronger than that of tetp and lacp, respectively.

Fig 1. Comparison on the strength of phlFp, tetp and lacp. Negative control represents BBa_E0240. Characterization was done using E. coli strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Results===
 Experiments on comparing the strength of <i>phlFp</i>, <i>tetp</i> and <i>lacp</i>, were performed. Results indicated that <i>phlFp</i> is the strongest among the three, with 1.96 times and 6 times stronger than that of <i>tetp</i> and <i>lacp</i>, respectively.
-<!--[[File:IGEM2016 HKUST sfGFPcharacterization.jpg|thumb|600px|center|<b>Fig 1. Comparison on the strength of <i>phlFp</i>, <i>tetp</i> and <i>lacp</i>.</b> Negative control represents BBa_E0240. Characterization was done using <i>E. coli</i> strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.]]-->
+[[File:IGEM2016 HKUST constructA.png|thumb|600px|center|<b>Fig 1. Comparison on the strength of <i>phlFp</i>, <i>tetp</i> and <i>lacp</i>.</b> Negative control represents BBa_E0240. Characterization was done using <i>E. coli</i> strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.]]
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