Difference between revisions of "Part:BBa K1884009"

Revision as of 09:52, 15 October 2016

PSAD Promoter+GalAD-CRY2+UAS+FNR(fumarate and nitrate reduction)+PSAD Terminator

The device we constructed this year is one of the functional plasmids in the light-mediated expression system,followed the example of yeast two-hybrid system. With BD-CIB1 which is constructed in the other device(BBa_K1884007), light-mediated expression system, followed the example of yeast two-hybrid system, will be activated and starting to transcript Ferredoxin-NADP(+)Reductase(BBa_K1884006) which is on the 3 prime end of Upstream activating sequence(BBa_K1884004) in green algae

Biology

PASD promoter(BBa_K1547005) is a plant promoter from the green algae Chlamydomonas reinhardtii. It is a high expression promoter that encodes for a ferrodoxin-binding protein of photosystem I.This year we decided to built the light-mediate-controlled system in green algae Chlamydomonas reinhardtii.Based on the origin of PSAD promoter, we thought it would be fully competible in our project.

AD-CRY2, a fusion protein, is for use in a yeast-two-hybrid system, and a VP16 DNA activating domian fused to its N terminus.In order to control DNA transcription by blue light, the system is based on a two-hybrid interaction in which a light-mediated protein brings together two halves of a split transcription factor. CRY2 will disconnected with CIB1 in the dark and halt the DNA transcription

UAS

FNR

PSAD terminator is a plant specific Terminator, and serves to increase expression of a gene placed upstream. It works in collaboration with PSAD promoter(BBa_K1547005). Both of these parts are activated by sunlight.

Usage

Sequence and Features