Difference between revisions of "Part:BBa K1913014:Design"

(→‎Design Notes)
 
Line 16: Line 16:
 
===Source===
 
===Source===
  
−
Bacillus thuringiensis var. tenebrionis
+
BBa_K081012 and the BBa_R0062 sequence.  
  
 
===References===
 
===References===

Latest revision as of 09:03, 15 October 2016


3-oxo-hexanoyl-HSL GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 720


Design Notes

This part was made from elongating part BBa_K081012 with primers: 5' ggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaatactagatgcg 3' and VR (5' attaccgcctttgagtgagc 3), and then subsequently with primers: 5' gaattcgcggccgcttctagagacctgtaggatcgtacaggtttacgcaagaaaatgg 3' and VR (5' attaccgcctttgagtgagc 3). These primers were based on the BBa_R0062 sequence.

Source

BBa_K081012 and the BBa_R0062 sequence.

References