Difference between revisions of "Part:BBa K2040101"

Revision as of 08:28, 15 October 2016

Fungal promoter PgpdA

PgpdA is the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter. This 2.1kb promoter containing a minimal gpdA promoter(BBa_K2040200) was isolated from vector pAN7-1(GenBank: Z32698.1).

Usage and Biology

To test the activity of gpdA promoter in entomopathogenic fungi Metarhizium.anisopliae, NYMU 2016 also constructed a composite part(BBa_K2040122) containing a mRFP coding region and put it downstream the promoter.

Results indicate this gpdA promoter

<image!>

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 903
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 137
Illegal XhoI site found at 693
Illegal XhoI site found at 1075
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1282
Illegal AgeI site found at 1430
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1109
Illegal SapI site found at 315

@@ Line 5: / Line 5: @@
 PgpdA is the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter. This 2.1kb promoter containing a minimal gpdA promoter(BBa_K2040200) was isolated from vector pAN7-1(GenBank: Z32698.1).
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+To test the activity of gpdA promoter in entomopathogenic fungi <i>Metarhizium.anisopliae</i>, NYMU 2016 also constructed a composite part(BBa_K2040122) containing a mRFP coding region and put it downstream the promoter.
+Results indicate this gpdA promoter
+<image!>
 <!-- -->