Difference between revisions of "Part:BBa K2145107"

Revision as of 04:22, 15 October 2016

GFP/RFP reporter (Reverse/Forward) with random 500bp spacer

This plasmid contains a GFP and an RFP reporter separated by a 500-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" (or a random sequence in) between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 825
Illegal AgeI site found at 937
1000
COMPATIBLE WITH RFC[1000]

Revision as of 04:12, 15 October 2016 (view source) Registry (Talk \| contribs)		Revision as of 04:22, 15 October 2016 (view source) Lchen (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K2145107 short</partinfo>		<partinfo>BBa_K2145107 short</partinfo>

−	This plasmid contains a GFP and an RFP reporter separated by a ~~532~~-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" (or a random sequence in) between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).	+	This plasmid contains a GFP and an RFP reporter separated by a 500-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" (or a random sequence in) between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).