Difference between revisions of "Part:BBa K2165004:Design"

 
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<partinfo>BBa_K2165002 short</partinfo>
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<partinfo>BBa_K2165004 short</partinfo>
  
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<partinfo>BBa_K2165002 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2165004 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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The Yeast Promoter Atlas was used to prevent altering bases that served a significant regulatory purpose (http://ypa.csbb.ntu.edu.tw/do?act=gene_by_kw&query=YHR053C#promoter_map). One base pair was substituted to eliminate the XbaI site in the middle of the sequence.
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The Yeast Promoter Atlas was used to prevent altering bases that served a significant regulatory purpose (http://ypa.csbb.ntu.edu.tw/do?act=gene_by_kw&query=YHR053C#promoter_map). Base pair number 106 was originally a guanine and was substituted with an adenine in the 5' to 3' direction to eliminate the XbaI site in the middle of the sequence.
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===Source===
 
===Source===

Revision as of 03:03, 15 October 2016


CUP1 yeast inducible promoter with RFC[10] restriction sites removed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Yeast Promoter Atlas was used to prevent altering bases that served a significant regulatory purpose (http://ypa.csbb.ntu.edu.tw/do?act=gene_by_kw&query=YHR053C#promoter_map). Base pair number 106 was originally a guanine and was substituted with an adenine in the 5' to 3' direction to eliminate the XbaI site in the middle of the sequence.

Source

The CUP1 promoter was identified in the 5' untranslated region of the metallothionein gene in Saccharomyces cerevisiae.

References