Difference between revisions of "Part:BBa K274001"
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Produces dark brown pigment output. The gene (melA) codes for a tyrosinase which produces the pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site. | Produces dark brown pigment output. The gene (melA) codes for a tyrosinase which produces the pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site. | ||
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| + | iGEM Concordia 2016 is currently characterizing this part in more efficient manner by improving the expression of melA through a point mutation. Refer to parts BBa_K2045004 and BBa_K2045005. | ||
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Revision as of 02:16, 15 October 2016
melanin pigment
Produces dark brown pigment output. The gene (melA) codes for a tyrosinase which produces the pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site.
iGEM Concordia 2016 is currently characterizing this part in more efficient manner by improving the expression of melA through a point mutation. Refer to parts BBa_K2045004 and BBa_K2045005.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1332
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1184
Illegal BsaI.rc site found at 489