Difference between revisions of "Part:BBa K2100008"

Revision as of 01:38, 15 October 2016

pENTR pPRE3

The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.

When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain. The DBD can then bind to the PREs on the promoter, recruiting transcriptional machinery and activating transcription.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 211
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 211
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 211
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 211
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 211
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 7
Illegal BsaI.rc site found at 225

@@ Line 5: / Line 5: @@
 The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.
-When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain.
+When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain. The DBD can then bind to the PREs on the promoter, recruiting transcriptional machinery and activating transcription.
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