Difference between revisions of "Part:BBa K2109108"

 
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<partinfo>BBa_K2109108 short</partinfo>
 
<partinfo>BBa_K2109108 short</partinfo>
  
this part is an improvement upon BBA_K1791000 from the Lethbridge iGEM 2015 submission. It contains a N terminal His tag on a modified MS2 coat protein sequence. The sequence has been codon optimized for Ecoli and modified via a V29I mutation and a 13 aa deletion with a Gly/Ala insertion. These mutations prevent capsid formation as well as dimerization of MS2 for more efficient yields during purification.  
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this part is an improvement upon BBA_K1791003 from the Lethbridge iGEM 2015 submission. It contains a N terminal His tag on a modified MS2 coat protein sequence. The sequence has been codon optimized for Ecoli and modified via a V29I mutation and a 13 aa deletion with a Gly/Ala insertion. These mutations prevent capsid formation as well as dimerization of MS2 for more efficient yields during purification.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:49, 14 October 2016


MS2 6XHis (V29I/d1FG)

this part is an improvement upon BBA_K1791003 from the Lethbridge iGEM 2015 submission. It contains a N terminal His tag on a modified MS2 coat protein sequence. The sequence has been codon optimized for Ecoli and modified via a V29I mutation and a 13 aa deletion with a Gly/Ala insertion. These mutations prevent capsid formation as well as dimerization of MS2 for more efficient yields during purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]