Difference between revisions of "Part:BBa K1529310"

Revision as of 23:06, 14 October 2016

Prhl(LR)

We newly developed the Prhl(LR) promoter (BBa_K1529310) by improving Prhl promoter (BBa_R0071) which is activated by C4HSL-RhlR complex (Fig. 1).
To characterize the function of this Prhl(LR) promoter, we constructed a part, Prhl(LR)-GFP (BBa_K1529311), by inserting the Prhl(LR) promoter, upstream of a GFP coding sequence.

Fig. 1. Our newly designed promoter

Fig. 2. Difference between LuxR and RhlR

By using the reporter cells that contain Prhl(LR)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL. (Fig. 3.)
We saw that our new Prhl(LR) promoter was actually activated by C4HSL through an induction assay

Fig. 3. Fluorescence intensity detected by flow cytometer

For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].

iGEM 2016 Tokyo_Tech

We improve BBa_K1529310 and design BBa_K1949060.

Sequence and Features