Difference between revisions of "Part:BBa K1981201"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This device is used to reflect AI-2 concentration in the nature or artificial environment. By co-culturing ‘AI-2 controllers’ with E.coli consisting of AI-2 Response Device A , we can know how the ‘controller cells’ manipulate the AI-2 level in the extracellular environment by simply measure the GFP expression level. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 22:51, 14 October 2016
Autoinducer-2 Response Device A
This composite part consists of the AI-2 (autoinducer-2) quorum sensor-inducible promoter BBa_K1981101, a GFP coding sequence BBa_E0040, a double terminator BBa_B0015. In AI-2 Response Device A, GFP expression is under the control of promoter, plsr. When phospho-AI-2 binds LsrR, expression of GFP ensues. The expression of GFP can directly response to the AI-2 level in the environment, which is an alternative way to reflect the AI-2 concentration in the nature or artificial environment.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 898