Difference between revisions of "Part:BBa K2016005"

Revision as of 20:23, 14 October 2016

Superfolder GFP under control of strong constitutive promoter and RyhB siRNA domain

Usage and Biology

This part consists of the superfolder GFP gene (sfgfp), with a RhyB binding sequence in its 5' UTR. RhyB is an srRNA that is expressed in E.coli and several other bacteria under low iron conditions. This results in sfGFP being downregulated in low iron conditions, which we see in the form of lower florescence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 514
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 38

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
 <p>This part consists of the superfolder GFP gene (<i>sfgfp</i>), with a RhyB binding sequence in its 5' UTR. RhyB is an srRNA that is expressed in <i>E.coli</i> and several other bacteria under low iron conditions. This results in sfGFP being downregulated in low iron conditions, which we see in the form of lower florescence.</p>
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2016005 SequenceAndFeatures</partinfo>
 ===Functional Parameters===
 <partinfo>BBa_K2016005 parameters</partinfo>