Difference between revisions of "Part:BBa I716013:Design"

 
 
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−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I716013 short</partinfo>
 
<partinfo>BBa_I716013 short</partinfo>
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{{BerkiGEM2007-Biobrick2.0Intro}}
 
{{BerkiGEM2007-Biobrick2.0Intro}}
 
<pre>
 
<pre>
−
ay008/9 on MG1665      349bp  (BglII, XhoI)
+
I716013 (pBca9145-yfbE-rbs-ATG)
 +
 
 +
ay008/9 on I716004        349bp  (BglII, XhoI)
 
into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
 
into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
 
prod I716013
 
prod I716013

Latest revision as of 18:53, 10 July 2007

yfbE/rbs/ATG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

I716013 (pBca9145-yfbE-rbs-ATG)

ay008/9 on I716004        349bp  (BglII, XhoI)
into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
prod I716013
---
ay008  cgacgAGATCTgctaacacgtaaactccac
ay009  gcgtgCTCGAGttaGGATCCcattgcttttcctt


Source

MG1665

References