Difference between revisions of "Part:BBa K1935022"

Revision as of 16:24, 14 October 2016

SET-16 promoter in Caenorhabditis elegans
Ceanorhabditis elegans is an organism with a free-methylated cytosine genome. We looked for a worm’s promoter able to be regulated as mammalian CpG island.
pSET-16 is a promoter of Caenorhabditis elegans classified as a HOT (Highly Occupied Target) regions. HOT regions have a rich G-C content that makes them similar to mammalian CpG islands. Some HOT promoters have been mentioned by Chen and one of them, SET-16 (chromosome 3: 13643462-13643761), controls a non-essential gene. That’s why we chose it as a target to test epiCRISPR whithout killing the worm.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1935022 short</partinfo>
+<br>
 Ceanorhabditis elegans is an organism with a free-methylated cytosine genome. We looked for a worm’s promoter able to be regulated as mammalian CpG island.
-<br> <br>
+<br>
 pSET-16 is a promoter of Caenorhabditis elegans classified as a HOT (Highly Occupied Target) regions. HOT regions have a rich G-C content that makes them similar to mammalian CpG islands.
 Some HOT promoters have been mentioned by Chen and one of them, SET-16 (chromosome 3: 13643462-13643761), controls a non-essential gene. That’s why we chose it as a target to test epiCRISPR whithout killing the worm.