Difference between revisions of "Part:BBa K1935022"

 
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<partinfo>BBa_K1935022 short</partinfo>
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Ceanorhabditis elegans is an organism with a free-methylated cytosine genome. We looked for a worm’s promoter able to be regulated as mammalian CpG island.
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<br> <br>
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pSET-16 is a promoter of Caenorhabditis elegans classified as a HOT (Highly Occupied Target) regions. HOT regions have a rich G-C content that makes them similar to mammalian CpG islands.
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Some HOT promoters have been mentioned by Chen and one of them, SET-16 (chromosome 3: 13643462-13643761), controls a non-essential gene. That’s why we chose it as a target to test epiCRISPR whithout killing the worm.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1935022 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1935022 parameters</partinfo>
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Revision as of 16:24, 14 October 2016


SET-16 promoter in Caenorhabditis elegans

Ceanorhabditis elegans is an organism with a free-methylated cytosine genome. We looked for a worm’s promoter able to be regulated as mammalian CpG island.

pSET-16 is a promoter of Caenorhabditis elegans classified as a HOT (Highly Occupied Target) regions. HOT regions have a rich G-C content that makes them similar to mammalian CpG islands. Some HOT promoters have been mentioned by Chen and one of them, SET-16 (chromosome 3: 13643462-13643761), controls a non-essential gene. That’s why we chose it as a target to test epiCRISPR whithout killing the worm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]