Difference between revisions of "Part:BBa K1969006"

 
 
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__NOTOC__
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<partinfo>BBa_K1969006 short</partinfo>
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It is a modified version of nucleotide binding alpha subunit of the heterotrimeric G protein (Gpa2), which originally interacts with the receptor Gpr1p and has signaling role in response to nutrients. it required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus. This modified version is aimed for improving signal transduction downstream heterologously expressed G protein coupled receptor in Saccharomyces cerevisiae. The original sequence of 5 amino acids at C terminus of Gpa2 is replaced by the corresponding sequence in human G alpha protein S1.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1969006 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1969006 parameters</partinfo>
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Latest revision as of 16:10, 14 October 2016


S. cerevisiae GPA2 with 5 amino acids from human G(s) alpha-subunit

It is a modified version of nucleotide binding alpha subunit of the heterotrimeric G protein (Gpa2), which originally interacts with the receptor Gpr1p and has signaling role in response to nutrients. it required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus. This modified version is aimed for improving signal transduction downstream heterologously expressed G protein coupled receptor in Saccharomyces cerevisiae. The original sequence of 5 amino acids at C terminus of Gpa2 is replaced by the corresponding sequence in human G alpha protein S1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4