Difference between revisions of "Part:BBa K1946005"

 
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<partinfo>BBa_K1946005 short</partinfo>
 
<partinfo>BBa_K1946005 short</partinfo>
  
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This part codes for the transcriptional regulatory protein XylR from Pseudomonas putida. It activates the TOL plasmid xyl operons in the presence of toluene, m-, p- and o-xylene.  
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This part codes for the transcriptional regulatory protein XylR from Pseudomonas putida. It activates the TOL plasmid xyl operons in the presence of toluene, m-, p- and o-xylene.
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Due to unavailability of older XylR coding part BBa_I723017 from iGEM07_Glasgow team, We newly synthesised it and codon optimised for E. Coli from XylR Uniprot amino acid sequence.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:36, 14 October 2016


XylR

This part codes for the transcriptional regulatory protein XylR from Pseudomonas putida. It activates the TOL plasmid xyl operons in the presence of toluene, m-, p- and o-xylene.

Due to unavailability of older XylR coding part BBa_I723017 from iGEM07_Glasgow team, We newly synthesised it and codon optimised for E. Coli from XylR Uniprot amino acid sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1665
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 937
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1082
    Illegal BsaI.rc site found at 1460