Difference between revisions of "Part:BBa K2075002"
| Line 8: | Line 8: | ||
and C-terminal ends. The team Heidelberg 2014 already used them for thei | and C-terminal ends. The team Heidelberg 2014 already used them for thei | ||
r circulation but we | r circulation but we | ||
| − | changed the sequence to make the circularization more accurate. They successful showed | + | changed the sequence to make the circularization more accurate. They successful showed that |
| − | + | ||
| − | + | ||
circularization enhances resistance against proteases and denaturation. We show t | circularization enhances resistance against proteases and denaturation. We show t | ||
hat circularization | hat circularization | ||
| − | with | + | with a TAL-effector makes them more solid and durable for everyday laboratory. |
| − | + | If you want more Information have a look of our results. The intein sequence have a size of 309 | |
| − | Information have a look of our results. The intein sequence have a size of 309 | + | |
bp. | bp. | ||
Latest revision as of 15:23, 14 October 2016
Intein
Inteins were integrated into proteins. They useful by the circularization
of a protein by ligating the N- and C-terminal ends. The team Heidelberg 2014 already used them for thei r circulation but we changed the sequence to make the circularization more accurate. They successful showed that circularization enhances resistance against proteases and denaturation. We show t hat circularization with a TAL-effector makes them more solid and durable for everyday laboratory.
If you want more Information have a look of our results. The intein sequence have a size of 309 bp.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]