Difference between revisions of "Part:BBa K1937007:Experience"

Revision as of 15:09, 14 October 2016

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Please enter how you used this part and how it worked out.

The antifungal property was tested against Talaromyces funiculosus. Briefly, plate were evenly inoculated with the fungus and a paper patches were added with either copper sulfate (positive control), water (negative control), Bacillus subtilis with pSBBS0K-mini (negative control, BBa_K1937001) or Bacillus subtilis with pSBBS0K-AF-A (BBa_K1937008 obtained from subcloning the BBa_K1937007part in the pSBBS0K-mini plasmid). Light but reproducible inhibition halo were observed after 3 days and still present after 8 days only when the Bacillus subtilis strain carry the antifungal plasmid.

Applications of BBa_K1937007

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UNIQb180b94f7a059808-partinfo-00000000-QINU UNIQb180b94f7a059808-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 how you used this part and how it worked out.
+The antifungal property was tested against Talaromyces funiculosus. Briefly, plate were evenly inoculated with the fungus and a paper patches were added with either copper sulfate (positive control), water (negative control), Bacillus subtilis with pSBBS0K-mini (negative control, BBa_K1937001) or Bacillus subtilis with pSBBS0K-AF-A (BBa_K1937008 obtained from subcloning the BBa_K1937007part in the pSBBS0K-mini plasmid). Light but reproducible inhibition halo were observed after 3 days and still present after 8 days only when the Bacillus subtilis strain carry the antifungal plasmid.
+[[File:BBa_K1937007-experience.png]]
 ===Applications of BBa_K1937007===