Difference between revisions of "Part:BBa K1935018:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
Mutagenesis was done to abolish illegal restriction site (Pst1)
+
Mutagenesis was done to abolish illegal restriction sites.
 +
<br>
 +
Pst1 restriction sites : 2214 pb, 2418 pb and 2448 pb  (G => C)
  
 
===Source===
 
===Source===

Latest revision as of 15:01, 14 October 2016


dead Cas9 (dCas9)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 2182
    Illegal NgoMIV site found at 2255
    Illegal NgoMIV site found at 2740
    Illegal NgoMIV site found at 3649
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutagenesis was done to abolish illegal restriction sites.
Pst1 restriction sites : 2214 pb, 2418 pb and 2448 pb (G => C)

Source

https://www.addgene.org/47106/sequences/

References