Difference between revisions of "Part:BBa K2039002"

Revision as of 15:00, 14 October 2016

Dimerization system FRB/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)

This part is the association of part K2039000 and K2039001.

The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria.

FKBP protein is linked to GFP 10 and FRB* protein is linked to GFP 11.

The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 716
Illegal NheI site found at 739
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1255
Illegal BamHI site found at 361
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 866
Illegal AgeI site found at 1040
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This part is the association of part K2039000 and K2039001.
-The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. FRB* protein is linked to GFP 11. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
+The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria.
+FKBP protein is linked to GFP 10 and FRB* protein is linked to GFP 11.
+The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
 <!-- Add more about the biology of this part here