Difference between revisions of "Part:BBa K1981006"
(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
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AI-2 “Consumer” was constructed by overexpressing the <i>lsrACDB</i>, <i>lsrFG</i> and <i>lsrK</i> genes in <i>Bacillus amyloliquefaciens LL3</i>.
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AI-2 “Consumer” was constructed by overexpressing the <i>lsrK</i> gene.
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<div>This part is originated from <i>Bacillus thuringiensis</i> serovar thuringiensis str. IS5056.</div>
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<div>This part is originated from <i>Escherichia coli</i> strain K12.</div>
  
 
===Characterizaion===
 
===Characterizaion===

Revision as of 14:33, 14 October 2016


lsrK

Autoinducer-2 kinase. Catalyzes the phosphorylation of autoinducer-2 (AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the lsr operon. Phosphorylates the ring-open form of (S)-4,5-dihydroxypentane-2,3-dione (DPD), which is the precursor to all AI-2 signaling molecules, at the C5 position. Required for the regulation of the lsr operon and many other genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 555
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 229
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

AI-2 “Consumer” was constructed by overexpressing the lsrK gene.

This part is originated from Escherichia coli strain K12.

Characterizaion