Difference between revisions of "Part:BBa K1913007:Experience"

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When an RBS library was added in front of the 434 cI repressor gene, colonies that contain part BBa_K1913006 (combining this part and BBa_K1913016) are found to color red. There is variation in the intensity of the red color, indicating that the RBS strength changed in the library has an effect on 434 cI protein levels, translating to downstream effects on promoters influenced by 434 cI repressor.
 
When an RBS library was added in front of the 434 cI repressor gene, colonies that contain part BBa_K1913006 (combining this part and BBa_K1913016) are found to color red. There is variation in the intensity of the red color, indicating that the RBS strength changed in the library has an effect on 434 cI protein levels, translating to downstream effects on promoters influenced by 434 cI repressor.
 
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<img src="https://static.igem.org/mediawiki/parts/0/0e/T--Wageningen_UR--imagenamehere.jpg">
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<figcaption>Figure 4. Close-up picture of culture plate with colonies containing the subpopulation two-operon plasmid (including the RBS library). The blue circled and (faintly) red circled colonies were selected to be used in separate plate reader experiments.</figcaption>
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===User Reviews===
 
===User Reviews===

Revision as of 14:12, 14 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1913007

When an RBS library was added in front of the 434 cI repressor gene, colonies that contain part BBa_K1913006 (combining this part and BBa_K1913016) are found to color red. There is variation in the intensity of the red color, indicating that the RBS strength changed in the library has an effect on 434 cI protein levels, translating to downstream effects on promoters influenced by 434 cI repressor.

T--Wageningen_UR--imagenamehere.jpg

User Reviews

UNIQa8736cb660b1d794-partinfo-00000000-QINU UNIQa8736cb660b1d794-partinfo-00000001-QINU