Difference between revisions of "Part:BBa K1913014"

 
Line 3: Line 3:
 
<partinfo>BBa_K1913014 short</partinfo>
 
<partinfo>BBa_K1913014 short</partinfo>
  
−
This is the Cry3Aa endotoxin with the arabinose inducable promoter. An RBS, TEV-site, and a His-tag have been added.
+
This part reports (with GFP production) the presence of 3-oxo-hexanoyl-HSL, the autoinducer in the lux quorum sensing system from <i>Vibrio fischeri</i>.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
3-oxo-hexanoyl-homoserine lactones (HSLs) are known to bind the 'lux-box' in the lux pR promoter (BBa_R0062). Binding of HSL in the promoter induces expression of downstream genes. HSLs function as signal molecules for inter bacterial communication: autoinducers. 3-oxo-hexanoyl-HSL specifically is the autoinducer in the lux quorum sensing system in <i>Vibrio fischeri</i>.
 +
This part consists of the lux pR promoter, an RBS
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 12:46, 14 October 2016


3-oxo-hexanoyl-HSL GFP reporter

This part reports (with GFP production) the presence of 3-oxo-hexanoyl-HSL, the autoinducer in the lux quorum sensing system from Vibrio fischeri.

Usage and Biology

3-oxo-hexanoyl-homoserine lactones (HSLs) are known to bind the 'lux-box' in the lux pR promoter (BBa_R0062). Binding of HSL in the promoter induces expression of downstream genes. HSLs function as signal molecules for inter bacterial communication: autoinducers. 3-oxo-hexanoyl-HSL specifically is the autoinducer in the lux quorum sensing system in Vibrio fischeri. This part consists of the lux pR promoter, an RBS


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 720