Difference between revisions of "Part:BBa I715013"

 
 
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<partinfo>BBa_I715013 short</partinfo>
 
<partinfo>BBa_I715013 short</partinfo>
  
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This part is designed to report the presence of functional Cre Recombinase. We plan to put it into plasmid pSB3K3 and cotransform it with BBa_I715012 to test the functionality of our Cre Recombinase with a hixC site inserted between amino acids 190 and 191.
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This part is in plasmid pSB3K3. We moved part BBa_S03737 to plasmid pSB3K3 to perform a cotransformation with with BBa_I715012 to test the functionality of our Cre Recombinase with a hixC site inserted between amino acids 190 and 191.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:56, 5 July 2007

pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB3K3)

This part is in plasmid pSB3K3. We moved part BBa_S03737 to plasmid pSB3K3 to perform a cotransformation with with BBa_I715012 to test the functionality of our Cre Recombinase with a hixC site inserted between amino acids 190 and 191.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1277
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1423
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1449
    Illegal NgoMIV site found at 1817
    Illegal NgoMIV site found at 1977
    Illegal AgeI site found at 257
    Illegal AgeI site found at 369
  • 1000
    COMPATIBLE WITH RFC[1000]