Difference between revisions of "Part:BBa K1918106:Design"

(→‎Design Notes)
(→‎Source)
 
Line 11: Line 11:
  
 
===Source===
 
===Source===
−
[pwx240(KpnI&XbaI)][pwx237(KpnI&XbaI)]
+
PCR from the existing plasmid in xie lab
  
 
===References===
 
===References===

Latest revision as of 12:36, 14 October 2016


CMV-Kozak-EBFP2N(1-154):IntN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 755
    Illegal SpeI site found at 83
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 755
    Illegal BamHI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


Design Notes

AIM: EBFP2N(1-154):IntN could be expressed in cell all time; ELEMENTS: EBFP2N(1-154):IntN, CMV

Source

PCR from the existing plasmid in xie lab

References