Difference between revisions of "Part:BBa K1985000"

Revision as of 12:35, 14 October 2016

mamP

The mamP gene from the magnetosome operon mamAB forms a protein homodimer. It is an iron-oxidase that contributes to the formation of magnetite by mediating on step in the process, the formation of iron(III)ferrihydrite.

Usage and Biology

Usage: The mamP gene produces a protein that can be used to transfer electrons to iron molecules, specfically, in vitro it can form iron(III)ferrihydrite. It can be used in combination with other mam genes to form an electron transport complex that should form magnetite (Fe(II)Fe(III)2O4) crystals when the cell is exposed to iron in solution. It was used to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.

Biology: MamP has been described as a cytochrome protein due to the presence two c-type cytochrome domains, with haem binding domains. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.

References

Siponen, Marina I. et al. "Structural Insight Into Magnetochrome-Mediated Magnetite Biomineralization". Nature 502.7473 (2013): 681-684.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 493
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1985000 short</partinfo>
+The mamP gene from the magnetosome operon mamAB forms a protein homodimer. It is an iron-oxidase that contributes to the formation of magnetite by mediating on step in the process, the formation of iron(III)ferrihydrite.
 ===Usage and Biology===
-Usage: The mamP gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the cell is exposed to iron in solution. It was to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.
+Usage: The mamP gene produces a protein that can be used to transfer electrons to iron molecules, specfically, in vitro it can form iron(III)ferrihydrite. It can be used in combination with other mam genes to form an electron transport complex that should form  magnetite (Fe(II)Fe(III)2O4) crystals when the cell is exposed to iron in solution. It was used to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.
 Biology:
-MamP is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.
+MamP has been described as a cytochrome protein due to the presence two c-type cytochrome domains, with haem binding domains. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.
 ===References===