Difference between revisions of "Part:BBa K2148014"

 
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gRNA guiding Cas9 to trnE2-psbH intergenic region of the Chlamydomonas reinhardtii chloroplast genome. The part has a psaA promoter, coded as PROMOTER/5UTR/NTAG/CDS/CTAG Phytobrick.
 
gRNA guiding Cas9 to trnE2-psbH intergenic region of the Chlamydomonas reinhardtii chloroplast genome. The part has a psaA promoter, coded as PROMOTER/5UTR/NTAG/CDS/CTAG Phytobrick.
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===Characterisation===
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===Usage and Biology===
 
===Usage and Biology===
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This part can be used with a system that manages to express Cas9 protein. It serves as the sgRNA which together with Cas9 can perfrom site-specific cuts on DNA that contains complementary sequences to the target RNA sequence in the sgRNA.
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<partinfo>BBa_K2148014 parameters</partinfo>
 
<partinfo>BBa_K2148014 parameters</partinfo>
 
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===References===
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Alex Reis, Ph.D., Bitesize Bio, Breton Hornblower, Ph.D., Brett Robb, Ph.D. and George Tzertzinis, Ph.D., New England Biolabs, Inc. CRISPR/Cas9 and Targeted Genome Editing: A New Era in Molecular Biology. NEB expressions Issue I, 2014
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John G Doench, Ella Hartenian, Daniel B Graham, Zuzana Tothova, Mudra Hegde, Ian Smith, Meagan Sullender, Benjamin L Ebert, Ramnik J Xavier & David E Root. Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nature Biotechnology 32, 1262–1267 (2014), doi:10.1038/nbt.3026
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Matthew H Larson, Luke A Gilbert, Xiaowo Wang, Wendell A Lim, Jonathan S Weissman & Lei S Qi. CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature Protocols 8, 2180–2196 (2013) doi:10.1038/nprot.2013.132

Revision as of 11:43, 14 October 2016


gRNA for CGG PAM

gRNA guiding Cas9 to trnE2-psbH intergenic region of the Chlamydomonas reinhardtii chloroplast genome. The part has a psaA promoter, coded as PROMOTER/5UTR/NTAG/CDS/CTAG Phytobrick.

Characterisation

Usage and Biology

This part can be used with a system that manages to express Cas9 protein. It serves as the sgRNA which together with Cas9 can perfrom site-specific cuts on DNA that contains complementary sequences to the target RNA sequence in the sgRNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Alex Reis, Ph.D., Bitesize Bio, Breton Hornblower, Ph.D., Brett Robb, Ph.D. and George Tzertzinis, Ph.D., New England Biolabs, Inc. CRISPR/Cas9 and Targeted Genome Editing: A New Era in Molecular Biology. NEB expressions Issue I, 2014

John G Doench, Ella Hartenian, Daniel B Graham, Zuzana Tothova, Mudra Hegde, Ian Smith, Meagan Sullender, Benjamin L Ebert, Ramnik J Xavier & David E Root. Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nature Biotechnology 32, 1262–1267 (2014), doi:10.1038/nbt.3026

Matthew H Larson, Luke A Gilbert, Xiaowo Wang, Wendell A Lim, Jonathan S Weissman & Lei S Qi. CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature Protocols 8, 2180–2196 (2013) doi:10.1038/nprot.2013.132