Difference between revisions of "Part:BBa K1918106:Experience"

(Applications of BBa_K1918106)
(Applications of BBa_K1918106)
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===Applications of BBa_K1918106===
 
===Applications of BBa_K1918106===
1. Use PCR to get the DNA fragment of CMV and EBFP2N(1-154):IntN
 
2. Ligate the fragment with vector
 
3. Heat shock transformation
 
4. Select colonies
 
5. Purification of plasmid DNA
 
7. Sequencing
 
8. Transfer into 293T cell
 
9. FACS
 
  
 
===User Reviews===
 
===User Reviews===

Revision as of 11:32, 14 October 2016


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Please enter how you used this part and how it worked out.

Applications of BBa_K1918106

User Reviews

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