Difference between revisions of "Part:BBa K1918102:Experience"

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===Applications of BBa_K1918102===
 
===Applications of BBa_K1918102===
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1. Use PCR to get the DNA fragment of TRE and EBFP2C(155-238)
 
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2. Ligate the fragment with vector
 
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3. Heat shock transformation
 
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4. Select colonies
 
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5. Purification of plasmid DNA
 
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7. Sequencing
 
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8. Transfer into 293T cell
 
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9. Use Dox to induce the expression of EBFP2C(155-238)
 
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10. FACS
 
  
 
===User Reviews===
 
===User Reviews===

Revision as of 11:30, 14 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1918102

User Reviews

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