Difference between revisions of "Part:BBa K1992003"
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<partinfo>BBa_K1992003 short</partinfo>
 
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Tar native RBS
  
 
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===Usage and Biology===
 
===Usage and Biology===
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In our project we focused on modify the Tar chemoreceptor originated from E.coli. In order to optimize the sensitivity of our system, we realize we first need to understand the native expression level of Tar receptor. Examination of the e.coli genome revealed that Tar expression monitoring by 𝝈28 promoter ,regulated by FNR protein as activator, and an unidentified RBS.
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As the promoter is very unconventional for biosynthesis and lab conditions uses we used a different induced promoter (rhlR). In addition we also use the native RBS of Tar in order to examine the effect it might has on the expression level and sensitivity of our system.
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In comparison to strong RBS usually use by iGEM teams (B0034) the Tar native RBS showed better results in the swarming assay experiment (figure).
  
 
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Revision as of 09:16, 14 October 2016


Tar chemoreceptor tagged with GFP

Tar native RBS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2324
    Illegal SapI.rc site found at 111