Difference between revisions of "Part:BBa K1937006:Design"

 
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The part include the pNagP promoter and its RBS (position 840,455 to 840,656 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping. This part is a subcloning of BBa_K1937005 pNagP part in pSBBS0K_mini plasmid (BBa_K1937001).
 
The part include the pNagP promoter and its RBS (position 840,455 to 840,656 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping. This part is a subcloning of BBa_K1937005 pNagP part in pSBBS0K_mini plasmid (BBa_K1937001).
  
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Here is a map of the part:
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[[File:BBa K1937005-map.png]]
  
  

Revision as of 09:07, 14 October 2016


pNagP-RFP in pSBBs0K-mini plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1278
    Illegal suffix found in sequence at 1306
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1278
    Illegal NheI site found at 222
    Illegal NheI site found at 1300
    Illegal SpeI site found at 1307
    Illegal PstI site found at 1321
    Illegal NotI site found at 7
    Illegal NotI site found at 1284
    Illegal NotI site found at 1314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1278
    Illegal BglII site found at 19
    Illegal BglII site found at 1020
    Illegal BglII site found at 5835
    Illegal BamHI site found at 1351
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1307
    Illegal EcoRI site found at 1278
    Illegal XbaI site found at 1293
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1278
    Illegal XbaI site found at 16
    Illegal XbaI site found at 1293
    Illegal SpeI site found at 1307
    Illegal PstI site found at 1321
    Illegal NgoMIV site found at 2898
    Illegal AgeI site found at 782
    Illegal AgeI site found at 894
    Illegal AgeI site found at 5446
    Illegal AgeI site found at 6408
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1076
    Illegal BsaI.rc site found at 2722
    Illegal BsaI.rc site found at 4161
    Illegal BsaI.rc site found at 6677
    Illegal SapI site found at 1639
    Illegal SapI.rc site found at 5659


Design Notes

The BBa_K1937006 part was designed to demonstrate the induction of pNagP by NAG. It belongs tothe antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). The part include the pNagP promoter and its RBS (position 840,455 to 840,656 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping. This part is a subcloning of BBa_K1937005 pNagP part in pSBBS0K_mini plasmid (BBa_K1937001).

Here is a map of the part: BBa K1937005-map.png


Source

In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate (Bertram et al., 2011: https://www.ncbi.nlm.nih.gov/pubmed/21602348). This NAG molecule is the major component of chitin, a characteristic molecule of fungi.

References