Difference between revisions of "Part:BBa K1919300"

 
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<partinfo>BBa_K1919300 short</partinfo>
 
<partinfo>BBa_K1919300 short</partinfo>
  
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This part contains a promoter, RBS, protein coading region and a terminator. The expression of the protein livJ in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been highly expressed in E.coli BL21 and E.coli DH5a.  
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LivJ encodes periplasmic binding protein for branched-chain amino acid ABC transporter which is encodes by operon livHMGF. The livJ gene product binds leucine, isoleucine, and valine. Please note that there is a short guide peptide on N terminal of livJ coding region, which is used to guide the precusor to be transported into periplasmic room. This device constructed in our team contains a promoter J23100, a RBS, livJ coding region and a terminator. The device can be transferred into E.coli and overexpress livJ directly.  The expression of the livJ RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been highly expressed in E.coli BL21 and E.coli DH5a.  
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LivJ is a crutial subunit of leucine transporter which is regulated by xxx. Overexpression of livJ can increase the untilizaiton of leucine in E.coli.
 
  
 
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Revision as of 09:04, 14 October 2016


livJ

LivJ encodes periplasmic binding protein for branched-chain amino acid ABC transporter which is encodes by operon livHMGF. The livJ gene product binds leucine, isoleucine, and valine. Please note that there is a short guide peptide on N terminal of livJ coding region, which is used to guide the precusor to be transported into periplasmic room. This device constructed in our team contains a promoter J23100, a RBS, livJ coding region and a terminator. The device can be transferred into E.coli and overexpress livJ directly. The expression of the livJ RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been highly expressed in E.coli BL21 and E.coli DH5a.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 382
    Illegal AgeI site found at 203
    Illegal AgeI site found at 485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 366