Difference between revisions of "Part:BBa K1918104:Experience"
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===Applications of BBa_K1918104=== | ===Applications of BBa_K1918104=== | ||
| + | 1. Use PCR to get the DNA fragment of TRE and IntC:EBFP2C(155-238) | ||
| + | 2. Ligate the fragment with vector | ||
| + | 3. Heat shock transformation | ||
| + | 4. Select colonies | ||
| + | 5. Purification of plasmid DNA | ||
| + | 7. Sequencing | ||
| + | 8. Transfer into 293T cell | ||
| + | 9. Use Dox to induce the expression of IntC:EBFP2C(155-238) | ||
| + | 10. FACS | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 07:51, 14 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1918104
1. Use PCR to get the DNA fragment of TRE and IntC:EBFP2C(155-238) 2. Ligate the fragment with vector 3. Heat shock transformation 4. Select colonies 5. Purification of plasmid DNA 7. Sequencing 8. Transfer into 293T cell 9. Use Dox to induce the expression of IntC:EBFP2C(155-238) 10. FACS
User Reviews
UNIQf8cbbd33bbe1c94f-partinfo-00000000-QINU UNIQf8cbbd33bbe1c94f-partinfo-00000001-QINU