Difference between revisions of "Part:BBa K1918103:Experience"
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===Applications of BBa_K1918103=== | ===Applications of BBa_K1918103=== | ||
| + | 1. Use PCR to get the DNA fragment of TRE and EBFP2N(1-154):IntN | ||
| + | 2. Ligate the fragment with vector | ||
| + | 3. Heat shock transformation | ||
| + | 4. Select colonies | ||
| + | 5. Purification of plasmid DNA | ||
| + | 7. Sequencing | ||
| + | 8. Transfer into 293T cell | ||
| + | 9. Use Dox to induce the expression of EBFP2N(1-154):IntN | ||
| + | 10. FACS | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 07:50, 14 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1918103
1. Use PCR to get the DNA fragment of TRE and EBFP2N(1-154):IntN 2. Ligate the fragment with vector 3. Heat shock transformation 4. Select colonies 5. Purification of plasmid DNA 7. Sequencing 8. Transfer into 293T cell 9. Use Dox to induce the expression of EBFP2N(1-154):IntN 10. FACS
User Reviews
UNIQ2e9679a06b6b9c74-partinfo-00000000-QINU UNIQ2e9679a06b6b9c74-partinfo-00000001-QINU