Difference between revisions of "Part:BBa K115001"

Revision as of 07:48, 14 October 2016

RNA thermometer (ROSE)

An [http://2008.igem.org/Team:TUDelft/Temperature_analysis#RNA_thermometer RNA thermometer] that can be used for temperature sensitive post-transcriptional regulation which is based on a RNA thermometer from Bradyrhizobium japonicum that initiates translation at 42°C.

The image shows the secondary structure of the part after ligation to a protein coding part, as predicted by [http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi RNAfold]. Notice that the 3' end, including the scar and the start codon, does not belong to the part. The blue region is the Shine Dalgarno sequence which is the ribosome binding site. The blue nucleotides are the ones that had to be changed in order to get the desired secondary structure after introduction of the scar.

Sequence and Features

3D Structure

(The following information is the contribution of SVCE_Chennai 2016)

3D Model when not ligated to any protein

Another representation of the 3D structure.

Secondary Structure when RNA thermometer is not ligated to any protein

References

SimRNAweb: a web server for RNA 3D structure modeling with optional restraints.