Difference between revisions of "Part:BBa K1918105:Experience"
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===Applications of BBa_K1918105=== | ===Applications of BBa_K1918105=== | ||
| + | 1. Use PCR to get the DNA fragment of CMV AND EBFP2C(155-238) | ||
| + | 2. Ligate the fragment with vector | ||
| + | 3. Heat shock transformation | ||
| + | 4. Select colonies | ||
| + | 5. Purification of plasmid DNA | ||
| + | 7. Sequencing | ||
| + | 8. Transfer into 293T cell | ||
| + | 9. FACS | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 07:47, 14 October 2016
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1918105
1. Use PCR to get the DNA fragment of CMV AND EBFP2C(155-238) 2. Ligate the fragment with vector 3. Heat shock transformation 4. Select colonies 5. Purification of plasmid DNA 7. Sequencing 8. Transfer into 293T cell 9. FACS
User Reviews
UNIQ87f23a0dcca42017-partinfo-00000000-QINU UNIQ87f23a0dcca42017-partinfo-00000001-QINU