Difference between revisions of "Part:BBa K1918106:Experience"
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===Applications of BBa_K1918106=== | ===Applications of BBa_K1918106=== | ||
| + | 1. Use PCR to get the DNA fragment of CMV and EBFP2N(1-154):IntN | ||
| + | 2. Ligate the fragment with vector | ||
| + | 3. Heat shock transformation | ||
| + | 4. Select colonies | ||
| + | 5. Purification of plasmid DNA | ||
| + | 7. Sequencing | ||
| + | 8. Transfer into 293T cell | ||
| + | 9. FACS | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 07:46, 14 October 2016
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1918106
1. Use PCR to get the DNA fragment of CMV and EBFP2N(1-154):IntN 2. Ligate the fragment with vector 3. Heat shock transformation 4. Select colonies 5. Purification of plasmid DNA 7. Sequencing 8. Transfer into 293T cell 9. FACS
User Reviews
UNIQff3bebbe6e622bc0-partinfo-00000000-QINU UNIQff3bebbe6e622bc0-partinfo-00000001-QINU