Difference between revisions of "Part:BBa K1918101:Experience"
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===Applications of BBa_K1918101=== | ===Applications of BBa_K1918101=== | ||
| + | 1. Use PCR to get the DNA fragment of TRE and EBFP2N(1-154) | ||
| + | 2. Ligate the fragment with vector | ||
| + | 3. Heat shock transformation | ||
| + | 4. Select colonies | ||
| + | 5. Purification of plasmid DNA | ||
| + | 7. Sequencing | ||
| + | 8. Transfer into 293T cell | ||
| + | 9. Use Dox to induce the expression of EBFP2N(1-154) | ||
| + | 10. FACS | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 07:36, 14 October 2016
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1918101
1. Use PCR to get the DNA fragment of TRE and EBFP2N(1-154) 2. Ligate the fragment with vector 3. Heat shock transformation 4. Select colonies 5. Purification of plasmid DNA 7. Sequencing 8. Transfer into 293T cell 9. Use Dox to induce the expression of EBFP2N(1-154) 10. FACS
User Reviews
UNIQcd200929f30bcfc3-partinfo-00000000-QINU UNIQcd200929f30bcfc3-partinfo-00000001-QINU