Difference between revisions of "Part:BBa K1897009:Design"

 
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===References===
 
===References===
 +
Fuqua, W. C., Winans, S. C., & Greenberg, E. P. (1994). Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. <i>Journal of bacteriology, 176</i>(2), 269.

Revision as of 07:21, 14 October 2016


LuxI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 869
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LuxI BBa_C0061 is shifted to after the HA tag.


Source

LuxI was obtained from biobrick part BBa_C0061, which was derived from Vibrio fischeri. The promoter was synthesized based on the sequence obtained from BBa_R0062. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.

References

Fuqua, W. C., Winans, S. C., & Greenberg, E. P. (1994). Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. Journal of bacteriology, 176(2), 269.