Difference between revisions of "Part:BBa K1897009"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part encodes LuxI that is under the control of pLuxR <partinfo>BBa_R0062</partinfo>. It can be used together with <partinfo>BBa_K1897008</partinfo> to create a quorum sensing system, where in the presence of high cell density, products under the control of pLuxR will be produced. | ||
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| + | The LuxI protein is a 193 amino acid protein that originates from <i>Vibrio fischeri</i>, and produces the autoinducer commonly known as N-3-(oxohexanoyl)homoserine lactone (AHL) (Fuqua <i>et al.</i>, 1994). The cell membrane is permeable to AHL, allowing AHL to accumulate in the surrounding environment (Fuqua <i>et al.</i>, 1994). In lower cell densities, AHL diffuses out of the cell down a concentration gradient, while in high cell densities, it is able to accumulate within the cell to turn on the transcrption of genes under the control of pLuxR (see: <partinfo>BBa_R0062</partinfo>) or pLuxL (see: <partinfo>BBa_R0063</partinfo>) (Fuqua <i>et al.</i>, 1994). | ||
Revision as of 07:20, 14 October 2016
LuxI
This part was made up of promoter pLuxR BBa_R0062, ribosome binding site (RBS) BBa_B0030, LuxI coding sequence BBa_C0061 and double terminator BBa_B0015.
Usage and Biology
This part encodes LuxI that is under the control of pLuxR BBa_R0062. It can be used together with BBa_K1897008 to create a quorum sensing system, where in the presence of high cell density, products under the control of pLuxR will be produced.
The LuxI protein is a 193 amino acid protein that originates from Vibrio fischeri, and produces the autoinducer commonly known as N-3-(oxohexanoyl)homoserine lactone (AHL) (Fuqua et al., 1994). The cell membrane is permeable to AHL, allowing AHL to accumulate in the surrounding environment (Fuqua et al., 1994). In lower cell densities, AHL diffuses out of the cell down a concentration gradient, while in high cell densities, it is able to accumulate within the cell to turn on the transcrption of genes under the control of pLuxR (see: BBa_R0062) or pLuxL (see: BBa_R0063) (Fuqua et al., 1994).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 869
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000COMPATIBLE WITH RFC[1000]